93351ESRecombinant Cynomolgus CD3E&CD3G/CD3 epsilon&a
Yeasen/翌圣生物 品牌
生产厂家厂商性质
上海市所在地
性能参数
表达区间及表达系统（Source） | Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with hFc tag at the C-terminal. It containsGln22-Asp117(CD3E)&Gln23-Thr113(CD3G). [Accession | Q95LI5.2(CD3E)&Q95LI7(CD3G)] |
分子量大小（Molecular Weight） | The protein has a predicted MW of 36.9 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation,the protein migrates to 44-46 kDa&46-48 kDa based on Tris-Bis PAGE result. |
内毒素（Endotoxin） | Less than 1EU per μg by the LAL method. |
纯度（Purity） | > 95% as determined by Tris-Bis PAGE and HPLC |
| 活性（Activity） | ELISA Data:Immobilized Cynomolgus CD3E&CD3G, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with EC50 of 51.2ng/ml determined by ELISA. |
制剂（Formulation） | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
重构方法（Reconstitution） | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
储存条件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
