裸鼠克拉拉细胞蛋白(CC16)ELISA试剂盒 ，英文名： Nude Mouse Clara cell protein,CC16 ELISA kit

国内统一规格：48T/96T
保质期：六个月
品牌：国内外ELISA试剂盒品牌
运输：包邮（中国大陆）
裸鼠克拉拉细胞蛋白(CC16)ELISA试剂盒包装形式：盒装（液体盒装）
ELISA实验操作步骤的一般常见方法
方法一）用于技术未知抗原的双抗体夹心法：
1）包被：用0.05M PH9.牰碳酸盐包被缓冲液将抗体稀释至蛋白质含量为1～10μg／ml。在每个聚苯乙烯板的反应孔中加0.1ml，4℃。次日，弃去孔内溶液，用洗涤缓冲液洗3次，每次3分钟。(简称洗涤，下同)。
2）加样：加一定稀释的待检样品0.1ml于上述已包被之反应孔中，置37℃孵育1小时。然后洗涤。(同时做空白孔，阴性对照孔及阳性对照孔)。
3）加酶标抗体：于各反应孔中，加入新鲜稀释的酶标抗体(经滴定后的稀释度)0.1ml。37℃孵育0.5～1小时，洗涤。
4）加底物液显色：于各反应孔中加入临时配制的TMB底物溶液0.1ml，37℃10～30分钟。
5）终止反应：于各反应孔中加入2M硫酸0.05ml。
6）裸鼠克拉拉细胞蛋白(CC16)ELISA试剂盒结果判定：可于白色背景上，直接用肉眼观察结果：反应孔内颜色越深，阳性程度越强，阴性反应为无色或极浅，依据所呈颜色的深浅，以“+”、“-”号表示。也可测O·D值：在ELISA技术仪上，于450nm(若以ABTS显色，则410nm)处，以空白对照孔调零后测各孔O·D值，若大于规定的阴性对照OD值的2.1倍，即为阳性。
方法二）用于技术未知抗体的间接法：　　　
用包被缓冲液将已知抗原稀释至1～10μg／ml，每孔加0.1ml，4℃。次日洗涤3次。加一定稀释的待检样品(未知抗体)0.1ml于上述已包被之反应孔中,置37℃孵育1小时,洗涤。(同时做空白、阴性及阳性孔对照)于反应孔中，加入新鲜稀释的酶标第二抗体(抗抗体)0.1ml，37℃孵育30-60分钟，洗涤,zui后一遍用DDW洗涤。其余步骤同“双抗体夹心法”的4、5、6。

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载脂蛋白C3(APOC3)技术试剂盒(酶联免疫吸附试验法) ELISA Kit for Apolipoprotein C3 (APOC3) 48T/96T Y10112 Homo sapiens (Human)

白介素7(IL7)技术试剂盒(酶联免疫吸附试验法) ELISA Kit for Interleukin 7 (IL7) 48T/96T Y10113 Rattus norvegicus (Rat)

AMP激活蛋白激酶β1(PRKAβ1)技术试剂盒(酶联免疫吸附试验法) ELISA Kit for Protein Kinase, AMP Activated Beta 1 (PRKAb1) 48T/96T Y10114 Homo sapiens (Human)

白介素6受体(IL6R)技术试剂盒(酶联免疫吸附试验法) ELISA Kit for Interleukin 6 Receptor (IL6R) 48T/96T Y10115 Homo sapiens (Human)

丙酮酸脱氢酶激酶同工酶4(PDK4)技术试剂盒(酶联免疫吸附试验法) ELISA Kit for Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4) 48T/96T Y10116 Rattus norvegicus (Rat)

丙酮酸脱氢酶激酶同工酶4(PDK4)技术试剂盒(酶联免疫吸附试验法) ELISA Kit for Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4) 48T/96T Y10117 Mus musculus (Mouse)

胞浆型磷脂酶A2(cPLA2)技术试剂盒(酶联免疫吸附试验法) ELISA Kit for Phospholipase A2, Cytosolic (cPLA2) 48T/96T Y10118 Rattus norvegicus (Rat)

ⅡD组磷脂酶A2(PLA2G2D)技术试剂盒(酶联免疫吸附试验法) ELISA Kit for Phospholipase A2, Group IID (PLA2G2D) 48T/96T Y10119 Rattus norvegicus (Rat)

三叶因子3(TFF3)技术试剂盒(酶联免疫吸附试验法) ELISA Kit for Trefoil Factor 3, Intestinal (TFF3) 48T/96T Y10120 Homo sapiens (Human)

防御素β1(DEFβ1)技术试剂盒(酶联免疫吸附试验法) ELISA Kit for Defensin Beta 1 (DEFb1) 48T/96T Y10121 Mus musculus (Mouse)

凝血因子Ⅷ(F8)技术试剂盒(酶联免疫吸附试验法) ELISA Kit for Coagulation Factor VIII (F8) 48T/96T Y10122 Homo sapiens (Human)

载脂蛋白F(APOF)技术试剂盒(酶联免疫吸附试验法) ELISA Kit for Apolipoprotein F (APOF) 48T/96T Y10123 Homo sapiens (Human)

载脂蛋白F(APOF)技术试剂盒(酶联免疫吸附试验法) ELISA Kit for Apolipoprotein F (APOF) 48T/96T Y10124 Mus musculus (Mouse)

磷脂酰丝氨酸(PS)技术试剂盒(酶联免疫吸附试验法) ELISA Kit for Phosphatidylserine (PS) 48T/96T Y10125 Homo sapiens (Human)

磷脂酰丝氨酸(PS)技术试剂盒(酶联免疫吸附试验法) ELISA Kit for Phosphatidylserine (PS) 48T/96T Y10126 Mus musculus (Mouse)

成纤维细胞生长因子10(FGF10)技术试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibroblast Growth Factor 10 (FGF10) 48T/96T Y10127 Rattus norvegicus (Rat)

白介素2(IL2)技术试剂盒(酶联免疫吸附试验法) ELISA Kit for Interleukin 2 (IL2) 48T/96T Y10128 Rhesus monkey (Simian)

白介素4(IL4)技术试剂盒(酶联免疫吸附试验法) ELISA Kit for Interleukin 4 (IL4) 48T/96T Y10129 Rhesus monkey (Simian)

载脂蛋白B48(APOB48)技术试剂盒(酶联免疫吸附试验法) ELISA Kit for Apolipoprotein B48 (APOB48) 48T/96T Y10130 Homo sapiens (Human)

受磷蛋白(PLN)技术试剂盒(酶联免疫吸附试验法) ELISA Kit for Phospholamban (PLN) 48T/96T Y10131 Homo sapiens (Human)

真核翻译起始因子3A(EIF3A)技术试剂盒(酶联免疫吸附试验法) ELISA Kit for Eukaryotic Translation Initiation Factor 3A (EIF3A) 48T/96T Y10132 Homo sapiens (Human)