荷兰Liposoma巨噬细胞清除剂Clodronate Liposome：氯膦酸盐脂质体是（Liposoma，CP-005-005）是一种可以利用巨噬细胞的内吞机制，将氯膦酸盐带入细胞内，诱导巨噬细胞凋亡的试剂。它是由荷兰阿姆斯特丹Vrije University 的Nico van Rooijen教授开发的，也叫做Clodronate Liposomes（氯膦酸二钠脂质体）。它是一种成熟，方便，经济的巨噬细胞清除方法，可以有效地清除动物体内不同组织部位和血液中的巨噬细胞。它是研究巨噬细胞功能的重要手段。荷兰Liposoma巨噬细胞清除剂频繁见刊于Cell，Nature和Science。譬如刚刚2024年12月4日的一篇Narure，发现了巨噬细胞的全新功能，也为我们研究康复运动，肌梭巨噬细胞Muscle Spindles MacroPhages（MSMP）氯膦酸盐脂质体巨噬细胞清除剂清除解决方案提供了参考。
论文信息：
论文题目： Macrophages excite muscle spindles with glutamate to bolster locomotion
期刊名称： Nature
时间期卷：Nature（2024）
在线时间：2024年12月4日
DOI：doi.org/10.1038/s41586-024-08272-5
货号：CP-005-005
规格：5ml+5ml
品牌：Liposoma
产地：荷兰
名称：Clodronate Liposomes and Control Liposomes
注射方式：Day1一次，Day3一次
注射方式：腹腔
检测部位：肌肉
荷兰Liposoma巨噬细胞清除剂Clodronate Liposomes在炎性疼痛模型巨噬细胞氯膦酸盐脂质体清除剂解决方案清除效果图：
荷兰Liposoma巨噬细胞清除剂Clodronate Liposomes在炎性疼痛模型巨噬细胞氯膦酸盐脂质体清除剂解决方案材料和方法：
Systemic depletion of macrophages
CSF1 inhibitor, BLZ945 (4 mg per mouse per day, S7725, Selleckchem) dissolved in sterile water with 20% 2-hydroxypropyl-β-cyclodextrin (H107, Sigma) or control with 20% 2-hydroxypropyl-β-cyclodextrin were delivered to the mice for four consecutive days by oral gavage. Clodronate liposomes or control liposomes (Liposoma) were injected into mice by intraperitoneal injection (200 μl per mouse per day) on days 1 and 3. Depleted or control mice were euthanized on day 4 (4 h after the last BLZ945 gavage) or day 11. Muscles together with spinal cords and sciatic nerves were dissected and freshly processed for FACS or fixed with 4% PFA and dehydrated with 30% sucrose for cryostat sectioning followed by immunostaining. Mice without or with macrophage depletion (oral gavage of control or BLZ945 in combination with control or clodronate liposomes) were used for behavioural assessments. Mouse hindlimbs were bilaterally assessed on day 11 after depletion. Animals were randomly and equally grouped before depletion treatments (15 biological replicates for each condition). All investigators were blind to the groups and treatments.
