<6550> 人晶状体上皮细胞（HLEpiC）提取于人晶状体组织，于原代冻存。
 
    组成哺乳动物晶状体的细胞包括两种类型：晶状体纤维细胞形成晶状体的绝大部分，单层上皮细胞覆盖在纤维前表面。眼睛晶状体的正常发育需要晶状体上皮细胞不断地分化，成熟。当这些细胞从晶状体中纬线部移行到晶状体内部后便产生透明的晶体蛋白，拉长形成晶状体纤维细胞，并且使细胞核和细胞器松散。晶状体上皮细胞分化的原因还不是很清楚，然而，决定晶状体上皮细胞分化的分子和细胞机制方面的研究已经有了一定进展。眼球液体中的生长因子将促进晶状体上皮细胞分化。例如：表皮生长因子促进有丝分裂，成纤维细胞生长因子，胰岛素生长因子和胰岛素促进它的迁移和分化。

    人晶状体上皮细胞（HLEpiC）提取于人晶状体组织，于原代冻存。每管含有细胞数>5×105 cells/ml，此细胞通过Fibronectin, Cytokeratin-18和-19 免疫荧光染色验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。
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储存：液氮

运输：干冰

用途：科研

The cells that make up the mammalian lens consist of two types: lens fiber cells, which form the bulk of the lens, and a monolayer of epithelial cells cover the anterior surface of the fibers. The normal development of the lens of the eye involves the progressive differentiation and maturation of the lens epithelial cells. As these cells migrate from the equatorial region of the lens into the interior of the lens, they produce the transparent crystallins, elongate to form lens fiber cells and loose their nuclei and other organelles [1]. The causes of lens epithelial cells differentiation are not well understood; however, some progress has been made in determining the underlying molecular and cellular processes of lens epithelial cell differentiation. This process can be promoted by growth factors present in the ocular fluids [2]. Some growth factors such as epidermal growth factor promote mitosis; others, such as basic fibroblast growth factor, insulin growth factor, and insulin promote cell migration and differentiation [3].

HLEpiC from ScienCell Research Laboratories are isolated from human lens. HLEpiC are cryopreserved at primary culture and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HLEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, cytokeratin-19 and fibronectin. HLEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HLEpiC are guaranteed to further culture at the conditions provided by ScienCell Research Laboratories.

Reference
[1]. Wagner, L. M. and Takemoto, D. J. （2001） PKCa and PKC? overexpression causes lentoid body formation in the N/N 1003A rabbit lens epithelial cell line. Molecular Vision 7: 138-144.
[2]. Lang, R. A. （1999） Which factors stimulate lens fiber cell differentiation in vivo? Invest Opthalmol Vis Sci 40:3075-8.
[3]. Leenders, W. P., van Genesen, S. T., Schoenmakers, J. G., van Zoelen, E. J., Lubsen, N. H. （1997） Synergism between temporally distinct growth factors: bFGF, insulin and lens cell differentiation. Mech Dev 67:193-201.