<7530>人间充质干细胞－脐带（HUMSC） 提取于人沃顿氏脐带，于*代冻存。
 
    间充质干细胞在成人的干细胞中数量恒定。它们有发育为成熟细胞的潜在能力而产生脂肪、软骨、骨骼和肌肉。这些特性与它们发育中的可塑性共同作用使人们对用间充质干细胞来替换受损组织的潜在能力产生了*的兴趣。分离于脐带脐带胶样组织中的间充质干细胞，在体外逐步培养在适于神经元生长的，含有sonic hedgehog和FGF-8的培养基中将转换为神经细胞和神经胶质，加入5-胞嘧啶将分化为心肌细胞，培养在适于心肌细胞生长的培养基中，将转化成脂肪和成骨质谱系。它们表达基质受体CD44和CD105，但不表达造血系统标志物CD34和大量地间充质干细胞标志物SH2和SH3。

    人间充质干细胞－脐带（HUMSC） 提取于人沃顿氏脐带，于*代冻存。每管含有细胞数>5×105 cells/ml，此细胞通过CD44, CD73免疫荧光染色验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。

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储存：液氮

运输：干冰

用途：科研

Mesenchymal stem cells （MSC） are a well-characterized population of adult stem cells. MSC have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. This property in combination with MSC's developmental plasticity have generated tremendous interest in the potential use of MSC to replace damaged tissues. MSC isolated from Wharton's jelly of umbilical cord were induced to transform into neurons and glia in vitro through stepwise culturing in neuron-conditioned medium, sonic hedgehog, and FGF-8 [1, 2]; to cardiomyocytes by treating then with 5-azacytidine or by culturing them in cardiomyocytes-conditioned medium; and to adipogenic and osteogenic lineages [3]. They express matrix receptors CD44 and CD105, but not hematopoietic lineage marker CD34, and a significant quantity of mesenchymal stem cell markers SH2 and SH3.

HUMSC from ScienCell Research Laboratories are isolated from Wharton's jelly of umbilical cord. HUMSC are cryopreserved at passage one culture and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HUMSC are characterized by immunofluorescent method with antibodies to CD44, CD73. HUMSC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HUMSC are guaranteed to further culture at the conditions provided by ScienCell Research Laboratories.

Reference
[1] Mitchell, K. E. et al. （2003） Matrix cells from Wharton's jelly form neurons and glia. Stem Cells 21:50-60.
[2] Fu, Y. S. et al. （2006） Conversion of human umbilical cord mesenchymal stem cells in Wharton's jelly to dopaminergic neurons in vitro: potential therapeutic application for Parkinsonium. Stem cells 24:115-124.
[3] Wang, H. S., et al., （2004） Mesenchymal stem cells in the Wharton's jelly of the human umbilical cord. Stem cells 22:1330-1337.