SOLARBIO/索莱宝 品牌
生产厂家厂商性质
北京市所在地
Anti-HSP70 Monoclonal Antibody
| 英文名称 | Anti-HSP70 Monoclonal Antibody |
|---|---|
| 宿主 | Mouse |
| 别名 | heat shock protein 70 |
| 应用 | WB IF |
| 稀释比例 | WB 1:1000-2000. IF 1:100-200. |
| 交叉反应 | Human Mouse Rat |
| 蛋白分子量 | 70kDa |
| Gene ID | 3303/3304 |
| 保存 | Store at -20°C. Avoid freeze / thaw cycles. |
| 储存液 | Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3. |
| 纯化方法 | Affinity purification |
| 亚型 | IgG1 |
| 免疫原 | A synthetic peptide coupled to KLH |
| 性状 | 液体 |
| Public Immunogen Range | A synthetic peptide coupled to KLH |
| Subcellular Locations | Cytoplasm |
| Swiss Prot | P0DMV8/P0DMV9 |
| 克隆号 | 3G10 |
| 克隆类型 | Monoclonal Antibody |
| 背景资料 | The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat shock proteins. Proteins with similar structure exist in virtually all living organisms. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. Hsp70 is usually in an ATP bound state. Hsp70 by itself is characterized by a very weak ATPase activity, such that spontaneous hydrolysis will not occur for many minutes. As newly synthesized proteins emerge from the ribosomes, the substrate binding domain of Hsp70 recognizes sequences of hydrophobic amino acid residues, and interacts with them. This spontaneous interaction is reversible, and in the ATP bound state Hsp70 may relatively freely bind and release peptides. However, the presence of a peptide in the binding domain stimulates the ATPase activity of Hsp70, increasing its normally slow rate of ATP hydrolysis. |
