试剂一、试剂二和试剂三均需避光保存。

试剂三变蓝绿色后不能再使用。

加入试剂三后必须立即混匀，否则显色不全。

取0.1g小鼠胰腺加入1mL提取液进行匀浆研磨，取上清后按照测定步骤操作，测得计算A测定管=0.324、A对照管=0.037、A空白管=0.022、A标准管=0.677，按样本质量计算酶活：

取兔血清后直接按照测定步骤操作，测得计算A测定管=0.190、A对照管=0.015、A空白管=0.022、A标准管=0.677，按血液体积计算酶活：

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