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人抗斑疹伤寒抗体酶免试剂盒,(anti-typhus-Ab)ELISA检测试剂盒 ,英文名:anti-typhus-Ab ELISA Kit ,英文缩写:anti-typhus-Ab ,常见试剂盒规格:96T/48T,产品别名:人抗斑疹伤寒抗体ELISA检测试剂盒,anti-typhus-Ab 检测试剂盒, anti-typhus-Ab 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。人抗斑疹伤寒抗体酶免试剂盒,(anti-typhus-Ab)ELISA检测试剂盒 在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
Endoglin蛋白(ENG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Endoglin (ENG)48T/96TY09635Mus musculus (Mouse)
细胞绒毛蛋白(CVL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cytovillin (CVL)48T/96TY09636Homo sapiens (Human)
通用转录因子ⅡE肽1(GTF2E1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for General Transcription Factor IIE, Polypeptide 1 (GTF2E1)48T/96TY09637Homo sapiens (Human)
杀伤细胞凝集素样受体亚家族C成员2(KLRC2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Killer Cell Lectin Like Receptor Subfamily C, Member 2 (KLRC2)48T/96TY09638Homo sapiens (Human)
烟碱型胆碱受体β2(CHRNβ2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cholinergic Receptor, Nicotinic, Beta 2 (CHRNb2)48T/96TY09639Homo sapiens (Human)
白介素1受体关联激酶1(IRAK1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Receptor Associated Kinase 1 (IRAK1)48T/96TY09640Homo sapiens (Human)
白介素1受体关联激酶2(IRAK2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Receptor Associated Kinase 2 (IRAK2)48T/96TY09641Homo sapiens (Human)
颗粒溶素(GNLY)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Granulysin (GNLY)48T/96TY09642Homo sapiens (Human)
白介素1受体关联激酶4(IRAK4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Receptor Associated Kinase 4 (IRAK4)48T/96TY09643Homo sapiens (Human)
白介素1受体关联激酶3(IRAK3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Receptor Associated Kinase 3 (IRAK3)48T/96TY09644Homo sapiens (Human)
黏膜地址素细胞黏附分子(MAdCAM1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1)48T/96TY09645Homo sapiens (Human)
肺部激活调节趋化因子(PARC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Pulmonary Activation Regulated Chemokine (PARC)48T/96TY09646Homo sapiens (Human)
维甲酸诱导基因1蛋白(RIG1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Retinoic Acid Inducible Gene 1 Protein (RIG1)48T/96TY09647Homo sapiens (Human)
趋化因子C-C-基元受体1(CCR1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Chemokine C-C-Motif Receptor 1 (CCR1)48T/96TY09648Homo sapiens (Human)
丝氨酸蛋白酶2(PRSS2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protease, Serine 2 (PRSS2)48T/96TY09649Homo sapiens (Human)
表面活性物质关联蛋白A(SPA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Surfactant Associated Protein A (SPA)48T/96TY09650Canis familiaris; Canine (Dog)
血清淀粉样P物质(SAP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Serum Amyloid P Component (SAP)48T/96TY09651Homo sapiens (Human)
抗糖脂抗体(AGA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Anti-Glycolipid Antibody (AGA)48T/96TY09652Homo sapiens (Human)
甘露糖受体C1(MRC1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Mannose Receptor C Type 1 (MRC1)48T/96TY09653Homo sapiens (Human)
甘露糖(MN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Mannose (MN)48T/96TY09654General
脂阿拉伯甘露聚糖(LAM)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lipoarabinomannan (LAM)48T/96TY09655Homo sapiens (Human)
吲哚胺2,3-双加氧酶(IDO)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Indoleamine-2,3-Dioxygenase (IDO)48T/96TY09656Homo sapiens (Human)
肽基脯氨酰异构酶F(PPIF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Peptidylprolyl Isomerase F (PPIF)48T/96TY09657Homo sapiens (Human)
Z-DNA结合蛋白1(ZBP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Z-DNA Binding Protein 1 (ZBP1)48T/96TY09658Homo sapiens (Human)
八聚体结合转录因子1(OCT1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Octamer Binding Transcription Factor 1 (OCT1)48T/96TY09659Homo sapiens (Human)
八聚体结合转录因子2(OCT2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Octamer Binding Transcription Factor 2 (OCT2)48T/96TY09660Homo sapiens (Human)
大型多功能肽酶7(LMP7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Large Multifunctional Peptidase 7 (LMP7)48T/96TY09661Homo sapiens (Human)
细胞色素P450家族成员21B(CYP21B)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cytochrome P450 21B (CYP21B)48T/96TY09662Homo sapiens (Human)
丝切蛋白1(CFL1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cofilin 1, Non Muscle (CFL1)48T/96TY09663Homo sapiens (Human)
淋巴毒素β(LTβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lymphotoxin Beta (LTb)48T/96TY09664Homo sapiens (Human)
干扰素调节因子1(IRF1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interferon Regulatory Factor 1 (IRF1)48T/96TY09665Homo sapiens (Human)
糖基化依赖性细胞黏附分子(GlyCAM1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glycosylation Dependent Cell Adhesion Molecule 1 (GlyCAM1)48T/96TY09666Homo sapiens (Human)
血小板因子4V1(PF4V1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Plaet Factor 4 Variant 1 (PF4V1)48T/96TY09667Homo sapiens (Human)
干扰素ω(IFNω)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interferon Omega (IFNw)48T/96TY09668Homo sapiens (Human)
粒细胞趋化蛋白2(GCP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Granulocyte Chemotactic Protein 2 (GCP2)48T/96TY09669Homo sapiens (Human)
IgG-Fc片段受体Ⅰ(FcγRⅠ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Receptor I For The Fc Region Of Immunoglobulin G (FcgRI)48T/96TY09670Homo sapiens (Human)
IgG-Fc片段受体Ⅱ(FcγRⅡ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Receptor II For The Fc Region Of Immunoglobulin G (FcgRII)48T/96TY09671Homo sapiens (Human)
细胞附着蛋白1(CYTH1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cytohesin 1 (CYTH1)48T/96TY09672Homo sapiens (Human)
IgE-Fc受体Ⅰ(FcεRⅠ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Receptor I For The Fc Region Of Immunoglobulin E (FceRI)48T/96TY09673Homo sapiens (Human)
IgE-Fc片段受体Ⅱ(FcεRⅡ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Receptor II For The Fc Region Of Immunoglobulin E (FceRII)48T/96TY09674Homo sapiens (Human)
细胞附着蛋白2(CYTH2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cytohesin 2 (CYTH2)48T/96TY09675Homo sapiens (Human)
单核细胞巨噬细胞分化关联蛋白(MMA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Monocyte To Macrophage Differentiation Associated Protein (MMA)48T/96TY09676Homo sapiens (Human)
磷脂酰肌醇蛋白聚糖2(GPC2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glypican 2 (GPC2)48T/96TY09677Homo sapiens (Human)
整合素β2(ITGβ2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Integrin Beta 2 (ITGb2)48T/96TY09678Homo sapiens (Human)
整合素连锁激酶(ILK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Integrin Linked Kinase (ILK)48T/96TY09679Homo sapiens (Human)
信号转导衔接分子1(STAM1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Signal Transducing Adaptor Molecule 1 (STAM1)48T/96TY09680Homo sapiens (Human)
干扰素调节因子3(IRF3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interferon Regulatory Factor 3 (IRF3)48T/96TY09681Homo sapiens (Human)
含钯蛋白(PALLD)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Palladin (PALLD)48T/96TY09682Homo sapiens (Human)
胱天蛋白酶1(CASP1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Caspase 1 (CASP1)48T/96TY09683Homo sapiens (Human)
酪氨酸激酶2(Tyk2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tyrosine Kinase 2 (Tyk2)48T/96TY09684Homo sapiens (Human)
各种类型ELISA测定时, 一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。