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人军团菌抗原酶免试剂盒,(LP Ag)ELISA检测试剂盒,英文名:LP Ag ELISA Kit,英文缩写:LP Ag ,常见试剂盒规格:96T/48T,产品别名:人军团菌抗原ELISA检测试剂盒,LP Ag 检测试剂盒,LP Ag 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。人军团菌抗原酶免试剂盒,(LP Ag)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
二胺氧化酶(DAO)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Diamine Oxidase (DAO)48T/96TY10085Rattus norvegicus (Rat)
高铁血红蛋白还原酶(MHBR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Methemoglobin Reductase (MHBR)48T/96TY10086Mus musculus (Mouse)
高铁血红蛋白还原酶(MHBR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Methemoglobin Reductase (MHBR)48T/96TY10087Rattus norvegicus (Rat)
环加氧酶2(PTGS2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Prostaglandin Endoperoxide Synthase 2 (PTGS2)48T/96TY10088Mus musculus (Mouse)
丝裂原激活蛋白激酶10(MAPK10)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Mitogen Activated Protein Kinase 10 (MAPK10)48T/96TY10089Rattus norvegicus (Rat)
神经调节素1(NRG1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neuregulin 1 (NRG1)48T/96TY10090Homo sapiens (Human)
表皮生长因子受体2(EGFR2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Epidermal Growth Factor Receptor 2 (EGFR2)48T/96TY10091Homo sapiens (Human)
金属硫蛋白2(MT2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Metallothionein 2 (MT2)48T/96TY10092Homo sapiens (Human)
微管蛋白β(TUBβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tubulin Beta (TUBb)48T/96TY10093Homo sapiens (Human)
G组着色性干皮病偶联因子(XPG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Xeroderma Pigmentosum, Complementation Group G (XPG)48T/96TY10094Homo sapiens (Human)
乳酸脱氢酶(LDH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lactate Dehydrogenase (LDH)48T/96TY10095Homo sapiens (Human)
白介素15(IL15)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 15 (IL15)48T/96TY10096Canis familiaris; Canine (Dog)
CD3d分子(CD3d)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cluster Of Differentiation 3d (CD3d)48T/96TY10097Mus musculus (Mouse)
CD19分子(CD19)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cluster Of Differentiation 19 (CD19)48T/96TY10098Mus musculus (Mouse)
癌胚抗原(CEA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Carcinoembryonic Antigen (CEA)48T/96TY10099Mus musculus (Mouse)
CD3d分子(CD3d)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cluster Of Differentiation 3d (CD3d)48T/96TY10100Rattus norvegicus (Rat)
凝血因子Ⅶ(F7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Coagulation Factor VII (F7)48T/96TY10101Homo sapiens (Human)
角蛋白13(KRT13)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Keratin 13 (KRT13)48T/96TY10102Homo sapiens (Human)
白介素5(IL5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 5 (IL5)48T/96TY10103Sus scrofa; Porcine (Pig)
高迁移率族核小体结合域蛋白1(HMGN1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for High Mobility Group Nucleosome Binding Domain Protein 1 (HMGN1)48T/96TY10104Homo sapiens (Human)
叉头框蛋白P3(FOXP3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Forkhead Box Protein P3 (FOXP3)48T/96TY10105Homo sapiens (Human)
乳酸脱氢酶(LDH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lactate Dehydrogenase (LDH)48T/96TY10106Mus musculus (Mouse)
白介素1受体关联激酶2(IRAK2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Receptor Associated Kinase 2 (IRAK2)48T/96TY10107Mus musculus (Mouse)
白介素1受体关联激酶2(IRAK2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Receptor Associated Kinase 2 (IRAK2)48T/96TY10108Rattus norvegicus (Rat)
小核糖核蛋白多肽D1(SNRPD1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Small Nuclear Ribonucleoprotein Polypeptide D1 (SNRPD1)48T/96TY10109Mus musculus (Mouse)
神经丝蛋白3(NEF3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neurofilament 3 (NEF3)48T/96TY10110Mus musculus (Mouse)
Apelin 36蛋白(AP36)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Apelin 36 (AP36)48T/96TY10111Homo sapiens (Human)
载脂蛋白C3(APOC3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Apolipoprotein C3 (APOC3)48T/96TY10112Homo sapiens (Human)
白介素7(IL7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 7 (IL7)48T/96TY10113Rattus norvegicus (Rat)
AMP激活蛋白激酶β1(PRKAβ1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protein Kinase, AMP Activated Beta 1 (PRKAb1)48T/96TY10114Homo sapiens (Human)
白介素6受体(IL6R)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 6 Receptor (IL6R)48T/96TY10115Homo sapiens (Human)
丙酮酸脱氢酶激酶同工酶4(PDK4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4)48T/96TY10116Rattus norvegicus (Rat)
丙酮酸脱氢酶激酶同工酶4(PDK4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4)48T/96TY10117Mus musculus (Mouse)
胞浆型磷脂酶A2(cPLA2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phospholipase A2, Cytosolic (cPLA2)48T/96TY10118Rattus norvegicus (Rat)
ⅡD组磷脂酶A2(PLA2G2D)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phospholipase A2, Group IID (PLA2G2D)48T/96TY10119Rattus norvegicus (Rat)
三叶因子3(TFF3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Trefoil Factor 3, Intestinal (TFF3)48T/96TY10120Homo sapiens (Human)
防御素β1(DEFβ1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Defensin Beta 1 (DEFb1)48T/96TY10121Mus musculus (Mouse)
凝血因子Ⅷ(F8)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Coagulation Factor VIII (F8)48T/96TY10122Homo sapiens (Human)
载脂蛋白F(APOF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Apolipoprotein F (APOF)48T/96TY10123Homo sapiens (Human)
载脂蛋白F(APOF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Apolipoprotein F (APOF)48T/96TY10124Mus musculus (Mouse)
磷脂酰丝氨酸(PS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phosphatidylserine (PS)48T/96TY10125Homo sapiens (Human)
磷脂酰丝氨酸(PS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phosphatidylserine (PS)48T/96TY10126Mus musculus (Mouse)
成纤维细胞生长因子10(FGF10)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibroblast Growth Factor 10 (FGF10)48T/96TY10127Rattus norvegicus (Rat)
白介素2(IL2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 2 (IL2)48T/96TY10128Rhesus monkey (Simian)
白介素4(IL4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 4 (IL4)48T/96TY10129Rhesus monkey (Simian)
载脂蛋白B48(APOB48)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Apolipoprotein B48 (APOB48)48T/96TY10130Homo sapiens (Human)
受磷蛋白(PLN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phospholamban (PLN)48T/96TY10131Homo sapiens (Human)
真核翻译起始因子3A(EIF3A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Eukaryotic Translation Initiation Factor 3A (EIF3A)48T/96TY10132Homo sapiens (Human)
43kDa胶原凝集素(CL43)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Collectin Of 43kDa (CL43)48T/96TY10133Bos taurus; Bovine (Cattle)
血管紧张素转化酶2(ACE2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Angiotensin I Converting Enzyme 2 (ACE2)48T/96TY10134Rattus norvegicus (Rat)
各种类型ELISA测定时,一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。