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人血吸虫酶免试剂盒,(schistosoma)ELISA检测试剂盒,英文名:schistosoma ELISA Kit,英文缩写:schistosoma ,常见试剂盒规格:96T/48T,产品别名:人血吸虫ELISA检测试剂盒,schistosoma 检测试剂盒,schistosoma 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。人血吸虫酶免试剂盒,(schistosoma)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
可溶性半乳糖凝集素3结合蛋白(LGALS3BP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP)48T/96TY09985Homo sapiens (Human)
微小染色体维持缺陷蛋白2(MCM2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Minichromosome Maintenance Deficient 2 (MCM2)48T/96TY09986Homo sapiens (Human)
S100钙结合蛋白A6(S100A6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for S100 Calcium Binding Protein A6 (S100A6)48T/96TY09987Homo sapiens (Human)
糖蛋白M6A(GPM6A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glycoprotein M6A (GPM6A)48T/96TY09988Homo sapiens (Human)
细胞分裂周期因子23(CDC23)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cell Division Cycle Protein 23 (CDC23)48T/96TY09989Homo sapiens (Human)
角质转谷氨酰胺酶(TGM1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transglutaminase 1, Keratinocyte (TGM1)48T/96TY09990Homo sapiens (Human)
髓细胞核分化抗原(MNDA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myeloid Cell Nuclear Differentiation Antigen (MNDA)48T/96TY09991Homo sapiens (Human)
干扰素调节因子8(IRF8)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interferon Regulatory Factor 8 (IRF8)48T/96TY09992Homo sapiens (Human)
涎福林蛋白(SPN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Sialophorin (SPN)48T/96TY09993Homo sapiens (Human)
类粘蛋白2(ORM2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Orosomucoid 2 (ORM2)48T/96TY09994Homo sapiens (Human)
磷脂爬行酶1(PLSCR1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phospholipid Scramblase 1 (PLSCR1)48T/96TY09995Homo sapiens (Human)
CD7分子(CD7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cluster Of Differentiation 7 (CD7)48T/96TY09996Homo sapiens (Human)
脑啡肽酶(NEP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neprilysin (NEP)48T/96TY09997Homo sapiens (Human)
受体相互作用丝氨酸苏氨酸激酶2(RIPK2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Receptor Interacting Serine Threonine Kinase 2 (RIPK2)48T/96TY09998Homo sapiens (Human)
Rho家族GTP酶1(RND1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Rho Family GTPase 1 (RND1)48T/96TY09999Homo sapiens (Human)
S100钙结合蛋白A8(S100A8)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for S100 Calcium Binding Protein A8 (S100A8)48T/96TY10000Homo sapiens (Human)
S100钙结合蛋白A9(S100A9)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for S100 Calcium Binding Protein A9 (S100A9)48T/96TY10001Homo sapiens (Human)
血清淀粉样蛋白A2(SAA2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Serum Amyloid A2 (SAA2)48T/96TY10002Homo sapiens (Human)
信号传导转录激活因子2(STAT2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Signal Transducer And Activator Of Transcription 2 (STAT2)48T/96TY10003Homo sapiens (Human)
CD5分子(CD5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cluster Of Differentiation 5 (CD5)48T/96TY10004Homo sapiens (Human)
激肽释放酶原(PK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Prekallikrein (PK)48T/96TY10005Homo sapiens (Human)
白介素21(IL21)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 21 (IL21)48T/96TY10006Rattus norvegicus (Rat)
转化生长因子β刺激蛋白22(TSC22)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transforming Growth Factor Beta Stimulated Protein Clone 22 (TSC22)48T/96TY10007Rattus norvegicus (Rat)
归巢关联细胞黏附分子(HCAM)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Homing Associated Cell Adhesion Molecule (HCAM)48T/96TY10008Homo sapiens (Human)
高分子量激肽原(HMWK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for High Molecular Weight Kininogen (HMWK)48T/96TY10009Homo sapiens (Human)
粘蛋白2(MUC2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Mucin 2 (MUC2)48T/96TY10010Mus musculus (Mouse)
粘蛋白7(MUC7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Mucin 7, Secreted (MUC7)48T/96TY10011Homo sapiens (Human)
棕榈酰化膜蛋白2(MPP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Membrane Protein, Palmitoylated 2 (MPP2)48T/96TY10012Homo sapiens (Human)
乙醇脱氢酶1(ADH1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alcohol Dehydrogenase 1 (ADH1)48T/96TY10013Homo sapiens (Human)
硒蛋白P1(SEPP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Selenoprotein P1, Plasma (SEPP1)48T/96TY10014Homo sapiens (Human)
维生素D结合蛋白(DBP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Vitamin D Binding Protein (DBP)48T/96TY10015Homo sapiens (Human)
簇集蛋白(CLU)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Clusterin (CLU)48T/96TY10016Rattus norvegicus (Rat)
尿嘧啶核苷磷酸化酶1(UPP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Uridine Phosphorylase 1 (UPP1)48T/96TY10017Homo sapiens (Human)
肝肾骨碱性磷酸酶(ALPL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alkaline Phosphatase, Liver/Bone/Kidney (ALPL)48T/96TY10018Rattus norvegicus (Rat)
β-位淀粉样前体蛋白裂解酶2(βACE2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Beta-Site APP Cleaving Enzyme 2 (bACE2)48T/96TY10019Homo sapiens (Human)
β-位淀粉样前体蛋白裂解酶2(βACE2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Beta-Site APP Cleaving Enzyme 2 (bACE2)48T/96TY10020Rattus norvegicus (Rat)
纤溶酶原(Plg)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Plasminogen (Plg)48T/96TY10021Rattus norvegicus (Rat)
白介素6受体(IL6R)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 6 Receptor (IL6R)48T/96TY10022Rattus norvegicus (Rat)
甲状旁腺激素(PTH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Parathyroid Hormone (PTH)48T/96TY10023Chicken (Gallus)
多功能蛋白聚糖(VS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Versican (VS)48T/96TY10024Homo sapiens (Human)
血管内皮生长因子受体1(VEGFR1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Vascular Endothelial Growth Factor Receptor 1 (VEGFR1)48T/96TY10025Homo sapiens (Human)
V-Jun肉瘤病毒癌基因同源物(JUN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN)48T/96TY10026Rattus norvegicus (Rat)
V-Jun肉瘤病毒癌基因同源物(JUN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN)48T/96TY10027Mus musculus (Mouse)
V-Fos-FBJ骨肉瘤病毒癌基因同源物(FOS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS)48T/96TY10028Rattus norvegicus (Rat)
V-Fos-FBJ骨肉瘤病毒癌基因同源物(FOS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS)48T/96TY10029Mus musculus (Mouse)
胰腺再生蛋白Ⅳ(REG4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Regenerating Islet Derived Protein 4 (REG4)48T/96TY10030Homo sapiens (Human)
肌钙蛋白T(TNT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Troponin T (TNT)48T/96TY10031Mus musculus (Mouse)
鞘氨醇(Sph)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Sphingosine (Sph)48T/96TY10032General
角蛋白17(KRT17)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Keratin 17 (KRT17)48T/96TY10033Homo sapiens (Human)
核因子κB(NFκB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nuclear Factor Kappa B (NFkB)48T/96TY10034Chicken (Gallus)
各种类型ELISA测定时,一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。