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人流行性乙型脑炎抗体IgG酶免试剂盒,(JE IgG)ELISA检测试剂盒,英文名:JE IgG ELISA Kit,英文缩写:JE IgG ,常见试剂盒规格:96T/48T,产品别名:人流行性乙型脑炎抗体IgGELISA检测试剂盒,JE IgG 检测试剂盒,JE IgG 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。人流行性乙型脑炎抗体IgG酶免试剂盒,(JE IgG)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
白介素21(IL21)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 21 (IL21)48T/96TY09885Homo sapiens (Human)
白介素28A(IL28A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 28A (IL28A)48T/96TY09886Homo sapiens (Human)
渗透性糖蛋白(Pgp)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Permeability Glycoprotein (Pgp)48T/96TY09887Homo sapiens (Human)
角蛋白10(KRT10)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Keratin 10 (KRT10)48T/96TY09888Homo sapiens (Human)
胸腺激活调节趋化因子(TARC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Thymus Activation Regulated Chemokine (TARC)48T/96TY09889Rattus norvegicus (Rat)
脂肪细胞型脂肪酸结合蛋白(FABP4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fatty Acid Binding Protein 4, Adipocyte (FABP4)48T/96TY09890Homo sapiens (Human)
白介素25(IL25)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 25 (IL25)48T/96TY09891Homo sapiens (Human)
白介素26(IL26)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 26 (IL26)48T/96TY09892Homo sapiens (Human)
血管内皮生长因子165(VEGF165)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Vascular Endothelial Growth Factor 165 (VEGF165)48T/96TY09893Homo sapiens (Human)
α1-抗胰蛋白酶(α1AT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alpha-1-Antitrypsin (a1AT)48T/96TY09894Homo sapiens (Human)
乳酸脱氢酶B(LDHB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lactate Dehydrogenase B (LDHB)48T/96TY09895Homo sapiens (Human)
乳酸脱氢酶B(LDHB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lactate Dehydrogenase B (LDHB)48T/96TY09896Rattus norvegicus (Rat)
乳酸脱氢酶B(LDHB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lactate Dehydrogenase B (LDHB)48T/96TY09897Mus musculus (Mouse)
血管生成素样蛋白3(ANGPTL3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Angiopoietin Like Protein 3 (ANGPTL3)48T/96TY09898Homo sapiens (Human)
白介素17B(IL17B)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 17B (IL17B)48T/96TY09899Rattus norvegicus (Rat)
WAP四二硫化物核心域蛋白1(WFDC1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for WAP Four Disulfide Core Domain Protein 1 (WFDC1)48T/96TY09900Mus musculus (Mouse)
胰淀素(IAPP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Islet Amyloid Polypeptide (IAPP)48T/96TY09901Oryctolagus cuniculus (Rabbit)
核糖核酸酶A3(RNASE3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ribonuclease A3 (RNASE3)48T/96TY09902Homo sapiens (Human)
TATA盒结合蛋白(TBP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for TATA Binding Protein (TBP)48T/96TY09903Homo sapiens (Human)
肿瘤坏死因子α(TNFα)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)48T/96TY09904Canis familiaris; Canine (Dog)
粒细胞巨噬细胞集落刺激因子(GMCSF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)48T/96TY09905Canis familiaris; Canine (Dog)
髓分化因子88(MyD88)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myeloid Differentiation Factor 88 (MyD88)48T/96TY09906Homo sapiens (Human)
法尼基二磷酸法尼基转移酶1(FDFT1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Farnesyl Diphosphate Farnesyltransferase 1 (FDFT1)48T/96TY09907Homo sapiens (Human)
T-细胞白血病/淋巴瘤蛋白1A(TCL1A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for T-Cell Leukemia/Lymphoma Protein 1A (TCL1A)48T/96TY09908Homo sapiens (Human)
糖盏蛋白(GC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glycocalicin (GC)48T/96TY09909Homo sapiens (Human)
Kruppel样因子5(KLF5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Kruppel Like Factor 5, Intestinal (KLF5)48T/96TY09910Homo sapiens (Human)
卷曲同源物8(FZD8)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Frizzled Homolog 8 (FZD8)48T/96TY09911Homo sapiens (Human)
信号传导转录激活因子1(STAT1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Signal Transducer And Activator Of Transcription 1 (STAT1)48T/96TY09912Homo sapiens (Human)
转化生长因子β3(TGFβ3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3)48T/96TY09913Sus scrofa; Porcine (Pig)
蛋白激酶Bβ(PKBβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protein Kinase B Beta (PKBb)48T/96TY09914Homo sapiens (Human)
白介素2受体α(IL2Rα)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 2 Receptor Alpha (IL2Ra)48T/96TY09915Sus scrofa; Porcine (Pig)
丝裂原激活蛋白激酶激酶6(MAP2K6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Mitogen Activated Protein Kinase Kinase 6 (MAP2K6)48T/96TY09916Homo sapiens (Human)
血清/糖皮质激素调节激酶2(SGK2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Serum/Glucocorticoid Regulated Kinase 2 (SGK2)48T/96TY09917Homo sapiens (Human)
表面活性物质关联蛋白D(SPD)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Surfactant Associated Protein D (SPD)48T/96TY09918Rattus norvegicus (Rat)
吡哆醛激酶(PDXK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Pyridoxal Kinase (PDXK)48T/96TY09919Homo sapiens (Human)
CD163分子(CD163)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cluster Of Differentiation (CD163)48T/96TY09920Homo sapiens (Human)
信号传导转录激活因子5B(STAT5B)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Signal Transducer And Activator Of Transcription 5B (STAT5B)48T/96TY09921Homo sapiens (Human)
生长分化因子2(GDF2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Growth Differentiation Factor 2 (GDF2)48T/96TY09922Homo sapiens (Human)
癌蛋白诱导转录物3(OIT3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Oncoprotein Induced Transcript 3 (OIT3)48T/96TY09923Homo sapiens (Human)
B-细胞淋巴瘤因子3(Bcl3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for B-Cell CLL/Lymphoma 3 (Bcl3)48T/96TY09924Homo sapiens (Human)
胱天蛋白酶4(CASP4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Caspase 4 (CASP4)48T/96TY09925Homo sapiens (Human)
激活STAT蛋白抑制因子1(PIAS1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protein Inhibitor Of Activated STAT 1 (PIAS1)48T/96TY09926Homo sapiens (Human)
信号传导转录激活因子3(STAT3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Signal Transducer And Activator Of Transcription 3 (STAT3)48T/96TY09927Homo sapiens (Human)
泛素D(UBD)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ubiquitin D (UBD)48T/96TY09928Homo sapiens (Human)
血小板型磷酸果糖激酶(PFKP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phosphofructokinase, Plaet (PFKP)48T/96TY09929Homo sapiens (Human)
己糖激酶3(HK3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Hexokinase 3, White Cell (HK3)48T/96TY09930Homo sapiens (Human)
色氨酰tRNA合成酶(WARS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tryptophanyl tRNA Synthetase (WARS)48T/96TY09931Homo sapiens (Human)
二氢嘧啶酶样蛋白3(DPYSL3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Dihydropyrimidinase Like Protein 3 (DPYSL3)48T/96TY09932Homo sapiens (Human)
肿瘤坏死因子配体超家族成员13(TNFSF13)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13)48T/96TY09933Homo sapiens (Human)
接触蛋白4(CNTN4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Contactin 4 (CNTN4)48T/96TY09934Rattus norvegicus (Rat)
各种类型ELISA测定时,一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。