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人白色念珠菌酶免试剂盒,(C.albicans)ELISA检测试剂盒,英文名:C.albicans ELISA Kit,英文缩写:C.albicans ,常见试剂盒规格:96T/48T,产品别名:人白色念珠菌ELISA检测试剂盒,C.albicans 检测试剂盒,C.albicans 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。人白色念珠菌酶免试剂盒,(C.albicans)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
心肌肌钙蛋白I(TNNI3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Troponin I Type 3, Cardiac (TNNI3)48T/96TY09835Oryctolagus cuniculus (Rabbit)
低氧诱导因子1α(HIF1α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a)48T/96TY09836Sus scrofa; Porcine (Pig)
乳铁传递蛋白(LTF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lactoferrin (LTF)48T/96TY09837Bos taurus; Bovine (Cattle)
钾氯协同转运蛋白4(KCC4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Potassium Chloride Cotransporters 4 (KCC4)48T/96TY09838Homo sapiens (Human)
钾氯协同转运蛋白2(KCC2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Potassium Chloride Cotransporters 2 (KCC2)48T/96TY09839Homo sapiens (Human)
钾氯协同转运蛋白3(KCC3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Potassium Chloride Cotransporters 3 (KCC3)48T/96TY09840Homo sapiens (Human)
趋化因子C-C-基元受体7(CCR7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Chemokine C-C-Motif Receptor 7 (CCR7)48T/96TY09841Homo sapiens (Human)
β2-微球蛋白(β2M)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Beta-2-Microglobulin (b2M)48T/96TY09842Rattus norvegicus (Rat)
三叶因子3(TFF3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Trefoil Factor 3, Intestinal (TFF3)48T/96TY09843Rattus norvegicus (Rat)
肌肉生长抑制素(MSTN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myostatin (MSTN)48T/96TY09844Homo sapiens (Human)
免疫球蛋白超家族成员16(IGSF16)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Immunoglobulin Superfamily, Member 16 (IGSF16)48T/96TY09845Homo sapiens (Human)
红细胞生成素(EPO)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Erythropoietin (EPO)48T/96TY09846Canis familiaris; Canine (Dog)
肌钙蛋白C(TNC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Troponin C (TNC)48T/96TY09847Rattus norvegicus (Rat)
连环蛋白β1(CTNNβ1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Catenin Beta 1 (CTNNb1)48T/96TY09848Mus musculus (Mouse)
乙酰辅酶A(A-CoA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Acetyl Coenzyme A (A-CoA)48T/96TY09849Homo sapiens (Human)
金属硫蛋白1(MT1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Metallothionein 1 (MT1)48T/96TY09850Rattus norvegicus (Rat)
α2-微球蛋白样蛋白1(α2ML1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alpha-2-Microglobulin Like Protein 1 (a2ML1)48T/96TY09851Homo sapiens (Human)
丙二酰辅酶A脱羧酶(MLYCD)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Malonyl Coenzyme A Decarboxylase (MLYCD)48T/96TY09852Rattus norvegicus (Rat)
抗突触前膜受体抗体(PsmRAb)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Anti-Presynaptic Membrane Receptor Antibody (PsmRAb)48T/96TY09853Homo sapiens (Human)
肌联蛋白(TTN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Titin (TTN)48T/96TY09854Homo sapiens (Human)
肉毒碱脂酰转移酶(CACT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Carnitine Acylcarnitine Translocase (CACT)48T/96TY09855Rattus norvegicus (Rat)
脂肪酸转运蛋白5(FATP5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fatty Acid Transport Protein 5 (FATP5)48T/96TY09856Rattus norvegicus (Rat)
乳酸脱氢酶A(LDHA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lactate Dehydrogenase A (LDHA)48T/96TY09857Rattus norvegicus (Rat)
柠檬酸合酶(CS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Citrate Synthase (CS)48T/96TY09858Rattus norvegicus (Rat)
糖皮质激素诱导肿瘤坏死因子受体(GITR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glucocorticoid Induced Tumor Necrosis Factor Receptor (GITR)48T/96TY09859Rattus norvegicus (Rat)
胰岛素(INS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Insulin (INS)48T/96TY09860Canis familiaris; Canine (Dog)
热休克蛋白47(HSP47)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heat Shock Protein 47 (HSP47)48T/96TY09861Rattus norvegicus (Rat)
溶菌酶(LZM)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lysozyme (LZM)48T/96TY09862Mus musculus (Mouse)
高尔基蛋白73(GP73)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Golgi Protein 73 (GP73)48T/96TY09863Homo sapiens (Human)
磷脂酰肌醇蛋白聚糖3(GPC3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glypican 3 (GPC3)48T/96TY09864Homo sapiens (Human)
黑素瘤细胞黏附分子(MCAM)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Melanoma Cell Adhesion Molecule (MCAM)48T/96TY09865Homo sapiens (Human)
AMP激活蛋白激酶β1(PRKAβ1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protein Kinase, AMP Activated Beta 1 (PRKAb1)48T/96TY09866Rattus norvegicus (Rat)
肺部激活调节趋化因子(PARC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Pulmonary Activation Regulated Chemokine (PARC)48T/96TY09867Mus musculus (Mouse)
转运蛋白1(TNPO1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transportin 1 (TNPO1)48T/96TY09868Mus musculus (Mouse)
胰岛素样生长因子结合蛋白6(IGFBP6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Insulin Like Growth Factor Binding Protein 6 (IGFBP6)48T/96TY09869Mus musculus (Mouse)
白介素12B(IL12B)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 12B (IL12B)48T/96TY09870Sus scrofa; Porcine (Pig)
促性腺激素释放激素受体(GnRHR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Gonadotropin Releasing Hormone Receptor (GnRHR)48T/96TY09871Rattus norvegicus (Rat)
孕酮受体(PGR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Progesterone Receptor (PGR)48T/96TY09872Rattus norvegicus (Rat)
激肽释放酶1(KLK1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Kallikrein 1 (KLK1)48T/96TY09873Homo sapiens (Human)
通用转录因子ⅡF肽1(GTF2F1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for General Transcription Factor IIF, Polypeptide 1 (GTF2F1)48T/96TY09874Homo sapiens (Human)
TATA框结合蛋白关联因子2(TAF2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for TATA Box Binding Protein Associated Factor 2 (TAF2)48T/96TY09875Homo sapiens (Human)
卵黄高磷蛋白(PV)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phosvitin (PV)48T/96TY09876Danio rerio (Zebrafish)
免疫球蛋白G2(IgG2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Immunoglobulin G2 (IgG2)48T/96TY09877Homo sapiens (Human)
细胞因子信号转导抑制因子3(SOCS3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Suppressors Of Cytokine Signaling 3 (SOCS3)48T/96TY09878Homo sapiens (Human)
整合素αM(ITGαM)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Integrin Alpha M (ITGaM)48T/96TY09879Homo sapiens (Human)
整合素αM(ITGαM)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Integrin Alpha M (ITGaM)48T/96TY09880Rattus norvegicus (Rat)
B-细胞激活因子(BAFF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for B-Cell Activating Factor (BAFF)48T/96TY09881Homo sapiens (Human)
高密度脂蛋白(HDL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for High Density Lipoprotein (HDL)48T/96TY09882Rattus norvegicus (Rat)
低密度脂蛋白(LDL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Low Density Lipoprotein (LDL)48T/96TY09883Rattus norvegicus (Rat)
甘油三酯(TG)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Triglyceride (TG)48T/96TY09884General
各种类型ELISA测定时,一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。