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人沙眼衣原体抗体酶免试剂盒,(CT)ELISA检测试剂盒,英文名:CT ELISA Kit,英文缩写:CT ,常见试剂盒规格:96T/48T,产品别名:人沙眼衣原体抗体ELISA检测试剂盒,CT 检测试剂盒,CT 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。人沙眼衣原体抗体酶免试剂盒,(CT)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
Ⅰ类主要组织相容性复合体E(MHCE)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Major Histocompatibility Complex Class I E (MHCE)48T/96TY09935Homo sapiens (Human)
谷氨酸受体相互作用蛋白1(GRIP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glutamate Receptor Interacting Protein 1 (GRIP1)48T/96TY09936Homo sapiens (Human)
干扰素调节因子4(IRF4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interferon Regulatory Factor 4 (IRF4)48T/96TY09937Homo sapiens (Human)
表皮转谷氨酰胺酶(TGM3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transglutaminase 3, Epidermal (TGM3)48T/96TY09938Homo sapiens (Human)
肌动蛋白束蛋白(FSCN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fascin (FSCN)48T/96TY09939Homo sapiens (Human)
核糖核酸酶A3(RNASE3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ribonuclease A3 (RNASE3)48T/96TY09940Mus musculus (Mouse)
羟甲基戊二酰辅酶A合酶(HMGCS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Hydroxymethylglutaryl Coenzyme A Synthase (HMGCS)48T/96TY09941Homo sapiens (Human)
胰腺再生蛋白Iα(REG1α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Regenerating Islet Derived Protein 1 Alpha (REG1a)48T/96TY09942Homo sapiens (Human)
抑瘤素M受体(OSMR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Oncostatin M Receptor (OSMR)48T/96TY09943Homo sapiens (Human)
抑制素βB(INHβB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Inhibin Beta B (INHbB)48T/96TY09944Homo sapiens (Human)
细胞色素b-561(CYB561)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cytochrome b-561 (CYB561)48T/96TY09945Homo sapiens (Human)
主要穹窿蛋白(MVP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Major Vault Protein (MVP)48T/96TY09946Homo sapiens (Human)
钙腔蛋白(CALU)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Calumenin (CALU)48T/96TY09947Homo sapiens (Human)
淀粉酶α(Amyα)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Amylase Alpha (Amya)48T/96TY09948Homo sapiens (Human)
白介素2受体α(IL2Rα)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 2 Receptor Alpha (IL2Ra)48T/96TY09949Homo sapiens (Human)
毒蕈碱型胆碱受体M3(CHRM3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cholinergic Receptor, Muscarinic 3 (CHRM3)48T/96TY09950Homo sapiens (Human)
小乳腺表皮粘蛋白(SBEM)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Small Breast Epithelial Mucin (SBEM)48T/96TY09951Homo sapiens (Human)
去氨精氨酸加压素(DDAVP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for 1-Desamino 8D Arginine Vasopressin (DDAVP)48T/96TY09952General
Ⅰ型前胶原氨基端原肽(PⅠNP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)48T/96TY09953Rattus norvegicus (Rat)
半胱氨酰白三烯受体2(CYSLTR2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cysteinyl Leukotriene Receptor 2 (CYSLTR2)48T/96TY09954Homo sapiens (Human)
丝裂原激活蛋白激酶激酶激酶激酶5(MAP4K5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Mitogen Activated Protein Kinase Kinase Kinase Kinase 5 (MAP4K5)48T/96TY09955Mus musculus (Mouse)
桩蛋白(Pax)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Paxillin (Pax)48T/96TY09956Homo sapiens (Human)
转化生长因子β2(TGFβ2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2)48T/96TY09957Chicken (Gallus)
转化生长因子β2(TGFβ2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2)48T/96TY09958Sus scrofa; Porcine (Pig)
巨噬细胞来源趋化因子(MDC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Derived Chemokine (MDC)48T/96TY09959Mus musculus (Mouse)
巨噬细胞炎性蛋白1α(MIP1α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a)48T/96TY09960Homo sapiens (Human)
巨噬细胞炎性蛋白1α(MIP1α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a)48T/96TY09961Mus musculus (Mouse)
巨噬细胞炎性蛋白1β(MIP1β)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 1 Beta (MIP1b)48T/96TY09962Homo sapiens (Human)
巨噬细胞炎性蛋白1β(MIP1β)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 1 Beta (MIP1b)48T/96TY09963Mus musculus (Mouse)
胱天蛋白酶11(CASP11)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Caspase 11 (CASP11)48T/96TY09964Mus musculus (Mouse)
巨噬细胞炎性蛋白3α(MIP3α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a)48T/96TY09965Homo sapiens (Human)
巨噬细胞炎性蛋白3α(MIP3α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a)48T/96TY09966Mus musculus (Mouse)
巨噬细胞炎性蛋白3α(MIP3α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a)48T/96TY09967Rattus norvegicus (Rat)
巨噬细胞炎性蛋白3β(MIP3β)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 3 Beta (MIP3b)48T/96TY09968Homo sapiens (Human)
巨噬细胞炎性蛋白3β(MIP3β)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 3 Beta (MIP3b)48T/96TY09969Mus musculus (Mouse)
基质金属蛋白酶1(MMP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 1 (MMP1)48T/96TY09970Homo sapiens (Human)
基质金属蛋白酶10(MMP10)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 10 (MMP10)48T/96TY09971Homo sapiens (Human)
基质金属蛋白酶13(MMP13)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 13 (MMP13)48T/96TY09972Homo sapiens (Human)
基质金属蛋白酶2(MMP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 2 (MMP2)48T/96TY09973Homo sapiens (Human)
基质金属蛋白酶2(MMP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 2 (MMP2)48T/96TY09974Mus musculus (Mouse)
基质金属蛋白酶3(MMP3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 3 (MMP3)48T/96TY09975Homo sapiens (Human)
基质金属蛋白酶3(MMP3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 3 (MMP3)48T/96TY09976Mus musculus (Mouse)
β-血小板球蛋白(βTG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Beta-Thromboglobulin (bTG)48T/96TY09977Homo sapiens (Human)
β-血小板球蛋白(βTG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Beta-Thromboglobulin (bTG)48T/96TY09978Rattus norvegicus (Rat)
β-血小板球蛋白(βTG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Beta-Thromboglobulin (bTG)48T/96TY09979Mus musculus (Mouse)
维生素E(VE)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Vitamin E (VE)48T/96TY09980General
组织金属蛋白酶抑制因子1(TIMP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)48T/96TY09981Sus scrofa; Porcine (Pig)
成纤维细胞生长因子7(FGF7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibroblast Growth Factor 7 (FGF7)48T/96TY09982Sus scrofa; Porcine (Pig)
免疫球蛋白E(IgE)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Immunoglobulin E (IgE)48T/96TY09983Sus scrofa; Porcine (Pig)
免疫球蛋白G(IgG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Immunoglobulin G (IgG)48T/96TY09984Sus scrofa; Porcine (Pig)
各种类型ELISA测定时,一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。