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人百日咳毒素酶免试剂盒,(PT)ELISA检测试剂盒,英文名:PT ELISA Kit,英文缩写:PT ,常见试剂盒规格:96T/48T,产品别名:人百日咳毒素ELISA检测试剂盒,PT 检测试剂盒,PT 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。人百日咳毒素酶免试剂盒,(PT)ELISA检测试剂盒 在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
叉头框蛋白P1(FOXP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Forkhead Box Protein P1 (FOXP1)48T/96TY09785Homo sapiens (Human)
叉头框蛋白O1(FOXO1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Forkhead Box Protein O1 (FOXO1)48T/96TY09786Homo sapiens (Human)
叉头框蛋白C1(FOXC1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Forkhead Box Protein C1 (FOXC1)48T/96TY09787Homo sapiens (Human)
叉头框蛋白O3(FOXO3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Forkhead Box Protein O3 (FOXO3)48T/96TY09788Homo sapiens (Human)
CD14分子(CD14)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cluster Of Differentiation 14 (CD14)48T/96TY09789Rattus norvegicus (Rat)
棕榈酰化膜蛋白2(MPP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Membrane Protein, Palmitoylated 2 (MPP2)48T/96TY09790Rattus norvegicus (Rat)
乙醇脱氢酶1(ADH1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alcohol Dehydrogenase 1 (ADH1)48T/96TY09791Rattus norvegicus (Rat)
颗粒酶K(GZMK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Granzyme K (GZMK)48T/96TY09792Homo sapiens (Human)
血管生成素4(ANGPT4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Angiopoietin 4 (ANGPT4)48T/96TY09793Homo sapiens (Human)
整合素αD(ITGαD)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Integrin Alpha D (ITGaD)48T/96TY09794Homo sapiens (Human)
脂多糖结合蛋白(LBP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lipopolysaccharide Binding Protein (LBP)48T/96TY09795Rattus norvegicus (Rat)
血管生成素4(ANGPT4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Angiopoietin 4 (ANGPT4)48T/96TY09796Rattus norvegicus (Rat)
血管生成素4(ANGPT4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Angiopoietin 4 (ANGPT4)48T/96TY09797Mus musculus (Mouse)
心肌肌钙蛋白I(TNNI3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Troponin I Type 3, Cardiac (TNNI3)48T/96TY09798Sus scrofa; Porcine (Pig)
碱性磷酸酶(ALP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alkaline Phosphatase (ALP)48T/96TY09799Rattus norvegicus (Rat)
CD6分子(CD6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cluster Of Differentiation 6 (CD6)48T/96TY09800Canis familiaris; Canine (Dog)
碱性磷酸酶(ALP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alkaline Phosphatase (ALP)48T/96TY09801Mus musculus (Mouse)
心钠肽(ANP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Atrial Natriuretic Peptide (ANP)48T/96TY09802Homo sapiens (Human)
凋亡诱导因子(AIF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Apoptosis Inducing Factor (AIF)48T/96TY09803Rattus norvegicus (Rat)
凋亡诱导因子(AIF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Apoptosis Inducing Factor (AIF)48T/96TY09804Mus musculus (Mouse)
粘蛋白5B(MUC5B)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Mucin 5 Subtype B (MUC5B)48T/96TY09805Homo sapiens (Human)
催乳素(PRL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Prolactin (PRL)48T/96TY09806Oryctolagus cuniculus (Rabbit)
蛋白酪氨酸磷酸酶受体N(PTPRN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protein Tyrosine Phosphatase Receptor Type N (PTPRN)48T/96TY09807Homo sapiens (Human)
亲环素B(CYPB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cyclophilin B (CYPB)48T/96TY09808Homo sapiens (Human)
抑制素结合蛋白(INHBP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Inhibin Binding Protein (INHBP)48T/96TY09809Rattus norvegicus (Rat)
溶菌酶(LZM)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lysozyme (LZM)48T/96TY09810Oryctolagus cuniculus (Rabbit)
干扰素β(IFNβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interferon Beta (IFNb)48T/96TY09811Chicken (Gallus)
核糖核酸酶A3(RNASE3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ribonuclease A3 (RNASE3)48T/96TY09812Rattus norvegicus (Rat)
血管紧张素Ⅱ受体1(AGTR1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Angiotensin II Receptor 1 (AGTR1)48T/96TY09813Homo sapiens (Human)
主要碱性蛋白(MBP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Major Basic Protein (MBP)48T/96TY09814Rattus norvegicus (Rat)
血管性血友病因子裂解蛋白酶(vWFcp)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Von Willebrand Factor Cleaving Protease (vWFCP)48T/96TY09815Rattus norvegicus (Rat)
激活素A受体Ⅰ(ACVR1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Activin A Receptor Type I (ACVR1)48T/96TY09816Rattus norvegicus (Rat)
白介素6(IL6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 6 (IL6)48T/96TY09817Canis familiaris; Canine (Dog)
白介素10(IL10)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 10 (IL10)48T/96TY09818Canis familiaris; Canine (Dog)
干扰素γ(IFNγ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interferon Gamma (IFNg)48T/96TY09819Canis familiaris; Canine (Dog)
颗粒酶K(GZMK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Granzyme K (GZMK)48T/96TY09820Rattus norvegicus (Rat)
Ⅲ型前胶原羧基端原肽(PⅢCP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Procollagen III C-Terminal Propeptide (PIIICP)48T/96TY09821Homo sapiens (Human)
Ⅱ型前胶原羧基端原肽(PⅡCP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Procollagen II C-Terminal Propeptide (PIICP)48T/96TY09822Homo sapiens (Human)
胰岛素样生长因子2受体(IGF2R)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Insulin Like Growth Factor 2 Receptor (IGF2R)48T/96TY09823Homo sapiens (Human)
组织内转化生长因子β结合蛋白2(LTBP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Latent Transforming Growth Factor Beta Binding Protein 2 (LTBP2)48T/96TY09824Homo sapiens (Human)
丝裂原激活蛋白激酶激活蛋白激酶3(MAPKAPK3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Mitogen Activated Protein Kinase Activated Protein Kinase 3 (MAPKAPK3)48T/96TY09825Homo sapiens (Human)
骨骼肌肌动蛋白α1(ACTα1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Actin Alpha 1, Skeletal Muscle (ACTa1)48T/96TY09826Homo sapiens (Human)
葡萄糖转运蛋白3(GLUT3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glucose Transporter 3 (GLUT3)48T/96TY09827Homo sapiens (Human)
转化生长因子β刺激蛋白22(TSC22)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transforming Growth Factor Beta Stimulated Protein Clone 22 (TSC22)48T/96TY09828Homo sapiens (Human)
前列腺素D合酶(PTGDS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Prostaglandin D2 Synthase, Hematopoietic (PTGDS)48T/96TY09829Homo sapiens (Human)
电子转移黄素蛋白β肽(ETFβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Electron Transfer Flavoprotein Beta Polypeptide (ETFb)48T/96TY09830Homo sapiens (Human)
磷酸甘油酸变位酶2(PGAM2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phosphoglycerate Mutase 2, Muscle (PGAM2)48T/96TY09831Homo sapiens (Human)
黑素瘤衍生亮氨酸拉链额外核因子(MLZE)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Melanoma Derived Leucine Zipper Extra Nuclear Factor (MLZE)48T/96TY09832Homo sapiens (Human)
结蛋白(Des)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Desmin (Des)48T/96TY09833Canis familiaris; Canine (Dog)
烟碱型胆碱受体β1(CHRNβ1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1)48T/96TY09834Homo sapiens (Human)
各种类型ELISA测定时, 一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。