小肠耶尔森菌核酸PCR检测试剂盒

广州健仑生物科技有限公司

广州健仑长期供应各种流感检测试剂，包括进口和国产的品牌，主要包括日本富士瑞必欧、日本生研、美国BD、美国NovaBios、美国binaxNOW、凯必利、广州创仑等。

产品名称：小肠耶尔森菌核酸PCR检测试剂盒；

 产品品牌：创仑；

 产品用途：本PCR-荧光探针法体外定性检测；

 产品规格：24T/盒； 

 保存条件：避光 -20度 保存。

    我司还提供各种流感病毒、副流感病毒、呼吸道合胞病毒、冠状病毒、轮状病毒、杆菌、链球菌、热带病毒等等核酸检测试剂盒（PCR-荧光探针法），随时欢迎您的来电

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

以下是我司提供的部分PCR产品

1. background of research

Grass carp (Ctenopharyngodon idella), also known as grass carp, grass carp, black, dronish, inhabits plains rivers and lakes. Aeromonas hydrophila (Aeromonas hydrophila) is widely distributed in all kinds of water bodies in nature, and is the primary pathogenic bacteria of various aquatic animals. For a long time, grass carp on Aeromonas hydrophila is susceptible, infected individuals have a higher mortality rate, resulting in large economic losses to the fishery resources. Aeromonas hydrophila invaded the fish body and then proliferated in the intestine first, then entered the liver, kidney and other tissues through the portal artery circulation, causing liver, kidney and other organs and blood diseases, followed by systemic symptoms. Then, from grass carp Aeromonas infection, the immune system of organism grass carp is how to make the response, gene mRNA level is few.

2. purpose of research

RNA-Seq research shows that a variety of fish species such as giant salamander, Qinghai Lake naked fish, turbot was Aeromonas hydrophila infection, the structure and function of immune related gene expression changes. In order to study from grass carp Aeromonas infection, immune response mechanism in the development of grass carp, Ocean University of China, Key Laboratory of aquatic resources and utilization of the party on the flying cloud infection before and after different time grass carp spleen by transcriptome sequencing.

3. research methods

The grass carp research sample collected from Jiangsu Wujiang state farms, breeding in circulating water with high k * * sterilization, three times a day feeding. The experimental group grass carp intraperitoneal injection of 100ul 2.7 * 107 CFU/mL of Aeromonas hydrophila, control group were injected with the same volume of grass carp PBS. The experimental group grass carp injection of Aeromonas hydrophila after 4, 8, 12, 24, 48 and 72 h respectively taken fish spleen tissue, and immediay frozen in liquid nitrogen and stored in the -80 C refrigerator. After each sample, the total RNA was extracted and sequenced.

4. analysis content and result explanation

4.1 sequence splicing and UniGene functional annotation

Through the analysis of RNA-seq non reference transcriptional group data, the sequence splicing was 52668 unigenes, the average length was 1072bp