12611ES24Hieff NGSTM Single-Indexed Adapter Kit for Illumin
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Hieff NGSTM Single-Indexed Adapter Kit for Illumina®, Set 1
产品信息
产品名称 | 产品编号 | 规格 | 储存 | 价格(元) |
Hieff NGSTM Single-Indexed Adapter Kit for Illumina®, Set 1 | 12611ES24 | 24 T | -20℃ | 1166.00 |
Hieff NGSTM Single-Indexed Adapter Kit for Illumina®, Set 1 | 12611ES96 | 96 T | -20℃ | 4366.00 |
产品描述
Hieff NGSTM Single-Indexed Adapter Kit for Illumina®, Set 1是Illumina®高通量测序平台文库构建试剂盒。试剂盒中包含Hieff NGSTM Adapter for Illumina,Hieff NGSTM Universal PCR Primer for Illumina以及12种Hieff NGSTM Index Primer for Illumina,适用于Illumina®高通量测序平台多样本文库构建。
产品组分
编号 | 组 分 | 产品编号/规格 | |
12611ES24 (24 T) | 12611ES96 (96 T) | ||
12611-A | Hieff NGSTM Adapter for Illumina (15 μM) | 80 μL | 320 μL |
12611-B | Hieff NGSTM Universal PCR Primer for Illumina (25 μM) | 60 μL | 240 μL |
12611-C1 | Hieff NGSTM Index 1 Primer for Illumina (25 μM) | 5 μL | 20 μL |
12611-C2 | Hieff NGSTM Index 2 Primer for Illumina (25 μM) | 5 μL | 20 μL |
12611-C3 | Hieff NGSTM Index 3 Primer for Illumina (25 μM) | 5 μL | 20 μL |
12611-C4 | Hieff NGSTM Index 4 Primer for Illumina (25 μM) | 5 μL | 20 μL |
12611-C5 | Hieff NGSTM Index 5 Primer for Illumina (25 μM) | 5 μL | 20 μL |
12611-C6 | Hieff NGSTM Index 6 Primer for Illumina (25 μM) | 5 μL | 20 μL |
12611-C7 | Hieff NGSTM Index 7 Primer for Illumina (25 μM) | 5 μL | 20 μL |
12611-C8 | Hieff NGSTM Index 8 Primer for Illumina (25 μM) | 5 μL | 20 μL |
12611-C9 | Hieff NGSTM Index 9 Primer for Illumina (25 μM) | 5 μL | 20 μL |
12611-C10 | Hieff NGSTM Index 10 Primer for Illumina (25 μM) | 5 μL | 20 μL |
12611-C11 | Hieff NGSTM Index 11 Primer for Illumina (25 μM) | 5 μL | 20 μL |
12611-C12 | Hieff NGSTM Index 12 Primer for Illumina (25 μM) | 5 μL | 20 μL |
产品应用
用于Illumina®高通量测序平台多样本文库构建。
运输与保存方法
冰袋运输。-20℃保存,有效期一年。
注意事项
1)Adapter为双链结构,请勿将其置于室温温度以上,以免发生解链、影响使用;
2)Index primer开盖前请短暂离心,使液体收集到管底,以免不同引物间交叉污染;
3)为了您的安全和健康,请穿实验服并戴一次性手套操作。
序列信息
1. Hieff NGSTM Adapter for Illumina
5´-ACACTCTTTCCCTACACGACGCTCTTCCGATC-s-T-3´
3´-CTGACCTCAAGTCTGCACACGAGAAGGCTAG-p-5´
【注】:-s-表示硫代,-p表示磷酸化。
2. Hieff NGSTM Universal PCR Primer for Illumina
5´-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC-s-T-3´
【注】:-s-表示硫代。
3. Hieff NGSTM Index Primer for Illumina
编号 | 名称 | 引物序列 | 测序时Index序列 |
12611-C1 | Hieff NGSTM Index 1 Primer for Illumina | 5´-CAAGCAGAAGACGGCATACGAGATCGTGATGTGA | ATCACG |
12611-C2 | Hieff NGSTM Index 2 Primer for Illumina | 5´-CAAGCAGAAGACGGCATACGAGATACATCGGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´ | CGATGT |
12611-C3 | Hieff NGSTM Index 3 Primer for Illumina | 5´-CAAGCAGAAGACGGCATACGAGATGCCTAAGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´ | TTAGGC |
12611-C4 | Hieff NGSTM Index 4 Primer for Illumina | 5´-CAAGCAGAAGACGGCATACGAGATTGGTCAGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´ | TGACCA |
12611-C5 | Hieff NGSTM Index 5 Primer for Illumina | 5´-CAAGCAGAAGACGGCATACGAGATCACTGTGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´ | ACAGTG |
12611-C6 | Hieff NGSTM Index 6 Primer for Illumina | 5´-CAAGCAGAAGACGGCATACGAGATATTGGCGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´ | GCCAAT |
12611-C7 | Hieff NGSTM Index 7 Primer for Illumina | 5´-CAAGCAGAAGACGGCATACGAGATGATCTGGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´ | CAGATC |
12611-C8 | Hieff NGSTM Index 8 Primer for Illumina | 5´-CAAGCAGAAGACGGCATACGAGATTCAAGTGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´ | ACTTGA |
12611-C9 | Hieff NGSTM Index 9 Primer for Illumina | 5´-CAAGCAGAAGACGGCATACGAGATCTGATCGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´ | GATCAG |
12611-C10 | Hieff NGSTM Index 10 Primer for Illumina | 5´-CAAGCAGAAGACGGCATACGAGATAAGCTAGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´ | TAGCTT |
12611-C11 | Hieff NGSTM Index 11 Primer for Illumina | 5´-CAAGCAGAAGACGGCATACGAGATGTAGCCGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´ | GGCTAC |
12611-C12 | Hieff NGSTM Index 12 Primer for Illumina | 5´-CAAGCAGAAGACGGCATACGAGATTACAAGGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´ | CTTGTA |
【注】:-s-表示硫代,下划线标记引物中的Index序列。
推荐使用
当您需要的Index数小于12个,推荐使用以下Index组合:
2个样品:Index 6,Index 12;
3个样品:Index 4,Index 6,Index 12;
6个样品:Index 2,Index 4,Index 5,Index 6,Index 7,Index 12。