12598ESHieff NGS® G-Type In-Situ DNA Binding Profili
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Hieff NGS™ DNA分选磁珠
面议Hieff NGSTM mRNA Isolation Master Kit
面议phi29 DNA Polymerase (10 U/μL)
¥585Recombinant Ten-Eleven Translocase
¥1555Recombinant Ten-Eleven Translocase (TET)14
¥15554×Multiplex PCR Master Mix for Pathogen
¥725Hieff Canace® Uracil+ High-Fidelity DNA polym
¥30154× Frag/Prime Buffer
¥102伴刀豆球蛋白A磁珠
¥1255Oligo dT 磁珠
¥805文库构建专用接头试剂盒
¥515DNA文库接头专用配套试剂盒set2
¥8200操作手册
CUT&Tag实验操作指南 点击下载>>
产品名称 | 产品编号 | 规格 |
Hieff NGS® G-Type In-Situ DNA Binding Profiling Library Prep Kit for Illumina® CUT&Tag试剂盒(pA/G-Tn5版) | 12598ES04 | 4 T |
12598ES12 | 12 T | |
12598ES48 | 48 T |
组分信息
组分编号 | 组分名称 | 12598ES04 | 12598ES12 | 12598ES48 | ||
BOX-I | 12598-A | ConA Beads | 40 μL | 120 μL | 480 μL | |
12598-B | DNA Selection Beads | 400 μL | 1.2 mL | 4.8 mL | ||
12598-C | Cell wash buffer | 3.6 mL | 10.8 mL | 43.2 mL | ||
12598-D | ConA bind buffer | 400 μL | 1.2 mL | 4.8 mL | ||
BOX-II | 12598-E | Protease Inhibitor Cocktail (EDTA-free) | 40 μL | 120 μL | 480 μL | |
12598-F | 5% Digitonin | 15 μL | 45 μL | 180 μL | ||
12598-G | 3 M NaCl | 80 μL | 240 μL | 960 μL | ||
12598-H | 50x Protein blocker | 4 μL | 12 μL | 48 μL | ||
12598-I | pA/G-Transposome Mix | 4 μL | 12 μL | 48 μL | ||
12598-J | 30x Activating buffer | 4 μL | 12 μL | 48 μL | ||
12598-K | 15x Terminate Solution | 8 μL | 24 μL | 96 μL | ||
12598-L | DNA Spike-in mix (5 pg/uL) | 4 μL | 12 μL | 48 μL | ||
12598-M | 30x Proteinase K | 4 μL | 12 μL | 48 μL | ||
12598-N | Primer Mix | 12 μL | 36 μL | 144 μL | ||
12598-O | 2×Ultima Amplification Mix | 100 μL | 300 μL | 1.2 mL | ||
12598-P | N5(N501) * | 4 μL | NA | NA | ||
12598-Q | N7(N701) * | 4 μL | NA | NA |
【注】:*测序时 Index 序列 填写时,测序平台为NovaSeq 6000 v1.0 reagents, MiSeq, HiSeq 2000/2500,则N501-CTCTCTAT,N701-TAAGGCGA。测序平台为NovaSeq 6000 v1.5 reagents,MiniSeq, NextSeq,HiSeq 3000/4000,则N501-ATAGAGAG,N701-TAAGGCGA。
BOX-I:2-8℃保存,BOX-II:-25~-15℃保存,有效期1年。